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- Introduction
- Features
- Publications
- Media
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| High-end exposure system for the direct exposure of cell culture inserts or Petri dishes developed on the basis of our patented linear CULTEX® glass modules – exposure modules of the 1st generation. The modular system consists of two main parts, the aerosol-guiding module and the sampling module housing the cell culture inserts. |
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| The aerosol-guiding module can be fitted tightly to the sampling module, thus realizing a close connection between the two parts before exposure. The sampling module houses three cell culture inserts (or Petri dishes), which can be separately supplied with medium. If required, the system can be upgraded with an to increase the deposition efficiency of sub-microsized particles. |
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| Design and handling |
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One modular system for 6.5 mm, 12 mm and 24 mm cell culture inserts as well as 36 mm Petri dishes (Ames assay). Use of commercially available cell culture inserts (COSTAR® and Falcon®) based on a flexible adapter system
Manufactured from boron silicate glass, polished stainless steel, silicone and PEEK
All components are autoclavable
Integration of the module into a specially designed rack system for higher stability and comfortable handling
Exchangeable emission ducts according to the cell culture insert type chosen
Tight connection to the aerosol guiding module by a specially designed locking device
Efficient connection to the generation device for the test atmosphere via the central aerosol inlet
Easy loading of the three positions by moving the sampling module on the rack system
Cell culture maintenance
Adjusted medium level
Controlled medium flow under each cell culture insert
Static, intermittent or continuous medium supply of the cultures via individual in- and outlets for each cell culture insert
Integrated water heating circuit, controlled by an external water bath |
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| Performance |
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Direct exposure of the biological test system
Test atmosphere entering the CULTEX® RFS via a central inlet from which three radial tubes guide the atmosphere to the cell culture inserts or Petri dishes for exposure
Individual exposure of the three positions via gas in- and outlets
Homogeneous distribution and deposition of particles on the surface of the test cultures
Dose-dependent exposure
Individual medium supply for all three cell culture inserts via separate medium inlets
Stability and robustness of the system lead to reproducible results
Measurement of various endpoints
Analysis of histopathological, biochemical, molecular biological and genetic endpoints
Direct exposure of mono- as well as co-cultures and tissue constructs of cell lines/primary cells of the respiratory tract or skin |
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Integration of an for efficient particle deposition without interfering with cell maintenance |
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Journal of Applied Toxicology 2014; 34:506-515
"Direct exposure at the air-liquid interface: evaluation of an in vitro approach for simulating inhalation of airborne substances"
Authors: J. Rach, J. Budde, N. Möhle, M. Aufderheide
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Chemico-Biological Interactions 2013; 206:479-490
"Use of the Cultex® Radial Flow System as an in vitro exposure method to assess acute pulmonary toxicity of fine dusts and nanoparticles with special focus on the intra- and inter-laboratory reproducibility"
Authors: D. Steinritz, N. Möhle, C. Pohl, M. Papritz, B. Stenger, A. Schmidt, C. J. Kirkpatrick, H. Thiermann, R. Vogel, S. Hoffmann, M. Aufderheide
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Experimental and Toxicologic Pathology 2013; 65:1183–1193
"A review of in vitro cigarette smoke exposure systems"
Authors: D. Thorne, J. Adamson
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Experimental and Toxicologic Pathology 2013; 65:683-688
"Detection of the cytotoxicity of water-insoluble fraction of cigarette smoke by direct exposure to cultured cells at an air-liquid interface"
Authors: H. Nara, Y. Fukano, T. Nishino, M. Aufderheide
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IVTIP 2013, Southhampton
"Prevalidation of the CULTEX® method (BMBF project 0315710): The air-liquid interface exposure of human lung cells in the CULTEX® Radial Flow System (RFS)"
Authors: N. Möhle, D. Steinritz, C. Pohl, S. Hoffmann, M. Aufderheide
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BioMed Research International 2013; 2013:73413
"The CULTEX® RFS: a comprehensive technical approach for the in vitro exposure of airway epithelial cells to the particulate matter at the air-liquid interface"
Authors: M. Aufderheide, B. Halter, N. Möhle, D. Hochrainer
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Eurotox 2011, Paris
"Importance of biomaterials testing prior to the construction of devices for in vitro techniques"
Authors: J. Rach, M. Aufderheide
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Eurotox 2011, Paris
"Prevalidation of the CULTEX® method: in vitro analysis of the acute toxicity of inhalable fine dusts and nanoparticles after direct exposure of cultivated human cells from the respiratory tract"
Authors: C. Pohl, N. Möhle, M. Papritz, D. Steinritz, U. Mohr, S. Hoffmann, C.J. Kirkpatrick, M. Aufderheide
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Journal of Water and Health 2011; 9:586-596
"Application of an optimized system for the well-defined exposure of human lung cells to trichloramine and indoor pool air"
Authors: C. Schmalz, H. G. Wunderlich, R. Heinze, F.H. Frimmel, C. Zwiener, T. Grummt
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Eurotox 2011, Paris
"Exposure of a human bronchial epithelial cell line with cigarette mainstream smoke in the CULTEX® Radial Flow System (RFS)"
Authors: D.-J. Budde, S. Scheffler, O. Krischenowski, M. Aufderheide
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Eurotox 2011, Paris
"Experimental study of aerosol cell exposure with an air-liquid interface"
Authors: M. Deutscher, N. Möhle, H. Bresch, S. Seeger, M. Aufderheide
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In vitro World Congress 2011, Montreal
"Pre-validation of the CULTEX® system assessing the acute inhalation toxicity of nanoparticles by direct exposure of cells via the air-liquid interface"
Authors: S. Hoffmann, N. Möhle, M. Papritz, C. Pohl, M. Aufderheide
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Eurotox 2011, Paris
"Prevalidation of the CULTEX® method: in vitro analysis of the acute toxicity of inhalable fine dusts and nanoparticles after direct exposure of cultivated human cells from the respiratory tract"
Authors: C. Pohl, N. Möhle, M. Papritz, D. Steinritz, U. Mohr, S. Hoffmann, C.J. Kirkpatrick, M. Aufderheide
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In vitro World Congress 2011, Montreal
"Need and Perspectives for the Implementation of Relevant In Vitro Methods in the Field of Inhalation Toxicology"
Authors: N. Möhle, J. Rach, S. Scheffler, D.-J. Budde, M. Aufderheide
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Analytical and Bioanalytical Chemistry 2011; 401:3213-3220
"Analytical in vitro approach for studying cyto- and genotoxic effects of particulate airborne material"
Authors: M. Aufderheide, S. Scheffler, N. Möhle, B. Halter, D. Hochrainer
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CULTEX® RFS
